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Water collection and filtration protocol using sterivex filters to collect environmental DNA from marine ecosystems. This collection and filtration protocol is used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.
Protocols used by AOML Omics labs, following the BeBOP-OBON standard
Nucleic acids extraction from the sterivex filters using the Qiagen DNeasy Blood and Tissue Kit with some modifications to the manufacturer’s protocol. These extraction protocols, adapted from Spens et al 2017, are used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.
This qPCR protocol is for amplifying and quantifying the nad2 gene in Dungeness crab (Metacarcinus magister).
Nucleic acids extraction from 47 mm disc filters using the Qiagen DNeasy Blood and Tissue Kit with some modifications to the manufacturer’s protocol. These extraction protocols, adapted from Spens et al 2017, are used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.
Protocol for running the Tourmaline 2 amplicon sequence processing workflow on data from assay Eukarya-18S-V9-Lane-Medlin
Protocol for preparing COI (Leray-Geller) amplicon libraries at Smithsonian NMNH
This protocol is for quantifying the concentration of DNA in the product of a DNA extraction.
Protocol for sampling water using Sterivex with Zymo ZR beads and DNA/RNA Shield
Water collection + EtOH subsampling protocol, plus subsequent filtration using disc filters and a vacuum manifold. This collection and filtration protocol is used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.
Water collection and filtration protocol using Smith-Root self preservng eDNA filters to collect environmental DNA from marine ecosystems. This collection and filtration protocol is used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.
Protocol for preparing 18S V9 (EMP) amplicon libraries for Michigan State RTSF
This PCR protocol is for amplifying the mitochondrial displacement loop (D-loop) gene also known as the mitochondial control region in marine mammals.
This PCR protocol is for amplifying the 16S rRNA v4-v5 region.
Organismal subsampling and Nucleic acids extraction from the tissue samples using the Qiagen DNeasy Blood and Tissue Kit with some modifications to the manufacturer’s protocol. These collection and extraction protocols used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.
Protocol for extraction of DNA from Sterivex filters using a KingFisher robot (BeBOP format)
Protocol for preparing 12S V5-V6 (MiFish) amplicon libraries for Michigan State RTSF
Protocol for extracting DNA from sediment trap samples using the Qiagen PowerSoil Pro Kit
This PCR protocol is for amplifying the 12S mitochondrial ribosomal RNA gene in vertebrates.
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