CysMAP

This repository contains code used in the manuscript:

Systematic cysteine scanning identifies a druggable pocket in oncogenic KRAS
van Tienen et al.

Overview

All processed mass spectrometry data required to reproduce the analyses are deposited on Figshare.

The Figshare record includes:

• BioPharma Finder PDF outputs derived from intact LC–MS raw files, which serve as input for the intact-mass analysis utilities.
• The CysMAP_ref.xlsx file containing required metadata for data annotation and processing, as well as processed data and a summary derived from the corresponding LC–MS data.

Raw LC–MS data are deposited separately at PRIDE and referenced in the associated manuscript.

Repository structure

• CysLibPool
  Code for proteome-wide (G)S-linker assignment and pooled variant design, used to evaluate mass separation constraints and linker distributions across CysMAP libraries.

• intact-mass
  Utilities for extraction and analysis of intact LC–MS data generated in CysMAP experiments.

Citation

If you use this code or associated data, please cite the corresponding manuscript.