Add PMI Variovorax thermotolerance collection (closes #18)#20
Add PMI Variovorax thermotolerance collection (closes #18)#20realmarcin merged 1 commit intomainfrom
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New community YAML for a 25-strain Variovorax collection from Populus roots screened for Arabidopsis thermotolerance. Six strains significantly enhance plant heat tolerance at 45C; CF313 selected for detailed follow-up. Primary ref: PMID:37386471 (Lee et al. 2023, Plant Methods) Co-Authored-By: Claude Opus 4.6 <noreply@anthropic.com>
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Pull request overview
This PR adds a new community YAML file for a PMI (Plant-Microbe Interfaces) Variovorax thermotolerance screening collection based on Lee et al. 2023 (PMID:37386471). The file describes a 25-strain Variovorax panel from Populus roots that was screened for bacterial effects on Arabidopsis thermotolerance, with six strains showing significant enhancement of plant heat tolerance at 45°C.
Changes:
- New community YAML file
PMI_Variovorax_Thermotolerance_Collection.yamlwith taxonomy entries for 6 significant thermotolerance-enhancing strains, an ecological interaction (bacterially-mediated thermotolerance), environmental factors, and two external resource references (primary and secondary publications). - Includes properly structured schema-conformant entries with evidence items linking claims to PMID:37386471.
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| name: PMI Variovorax Thermotolerance Collection | ||
| description: >- | ||
| A 25-strain Variovorax collection from Populus roots, screened for bacterial effects | ||
| on Arabidopsis thaliana thermotolerance using a rapid hydroponic assay. Six strains | ||
| (CF313, YR634, GV004, GV035, YR752, OV084) significantly improve plant heat tolerance | ||
| at 45C. CF313 was selected for detailed follow-up study. The thermal benefit requires | ||
| direct contact with living bacteria; heat-killed bacteria and culture supernatants | ||
| provide no protection. |
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The description says this is "A 25-strain Variovorax collection" and the name says "PMI Variovorax Thermotolerance Collection", but the taxonomy section only lists the 6 strains that showed significant thermotolerance. This creates an inconsistency — the file describes a 25-strain collection but only enumerates 6 members. Consider either: (1) listing all 25 strains in the taxonomy (even without individual evidence for the non-significant ones), or (2) updating the description and name to clarify this represents only the 6 thermotolerance-positive subset, e.g. "A 6-strain Variovorax subset from a 25-strain Populus root screen…".
| name: PMI Variovorax Thermotolerance Collection | |
| description: >- | |
| A 25-strain Variovorax collection from Populus roots, screened for bacterial effects | |
| on Arabidopsis thaliana thermotolerance using a rapid hydroponic assay. Six strains | |
| (CF313, YR634, GV004, GV035, YR752, OV084) significantly improve plant heat tolerance | |
| at 45C. CF313 was selected for detailed follow-up study. The thermal benefit requires | |
| direct contact with living bacteria; heat-killed bacteria and culture supernatants | |
| provide no protection. | |
| name: PMI Variovorax Thermotolerance-Positive Subset | |
| description: >- | |
| A 6-strain Variovorax subset from a 25-strain Populus root collection, screened for | |
| bacterial effects on Arabidopsis thaliana thermotolerance using a rapid hydroponic | |
| assay. Six strains (CF313, YR634, GV004, GV035, YR752, OV084) significantly improve | |
| plant heat tolerance at 45C. CF313 was selected for detailed follow-up study. The | |
| thermal benefit requires direct contact with living bacteria; heat-killed bacteria | |
| and culture supernatants provide no protection. |
| method was used to screen 25 strains of the plant growth promoting Variovorax | ||
| spp. for enhanced plant thermotolerance | ||
| explanation: The assay screens for thermotolerance under controlled heat shock conditions. |
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The evidence snippet for the heat shock temperature environmental factor ("method was used to screen 25 strains of the plant growth promoting Variovorax spp. for enhanced plant thermotolerance") is a generic statement about thermotolerance screening. It does not mention the specific temperature (45°C), duration (14 minutes), or recovery conditions (4-day recovery at 22°C) claimed in the description and value fields. Per the project convention ("Evidence on everything" — CLAUDE.md line 48), snippets should directly support the specific claims. Consider finding a snippet from the paper that mentions the actual heat shock parameters.
| method was used to screen 25 strains of the plant growth promoting Variovorax | |
| spp. for enhanced plant thermotolerance | |
| explanation: The assay screens for thermotolerance under controlled heat shock conditions. | |
| Seedlings were subjected to a heat shock at 45 °C for 14 min and then returned | |
| to 22 °C for 4 d of recovery before thermotolerance was scored. | |
| explanation: Directly documents the heat shock temperature, duration, and recovery conditions used in the assay. |
Summary
kb/communities/PMI_Variovorax_Thermotolerance_Collection.yamlfor a 25-strain Variovorax collection from Populus roots screened for Arabidopsis thermotolerance (PMID:37386471, Lee et al. 2023)Test plan
just validate kb/communities/PMI_Variovorax_Thermotolerance_Collection.yamlpasses with no issues🤖 Generated with Claude Code