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Add PMI Variovorax thermotolerance collection (closes #18)#20

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realmarcin merged 1 commit intomainfrom
add-pmi-variovorax-thermotolerance
Mar 6, 2026
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Add PMI Variovorax thermotolerance collection (closes #18)#20
realmarcin merged 1 commit intomainfrom
add-pmi-variovorax-thermotolerance

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@cmungall cmungall commented Mar 5, 2026

Summary

  • Add new community YAML kb/communities/PMI_Variovorax_Thermotolerance_Collection.yaml for a 25-strain Variovorax collection from Populus roots screened for Arabidopsis thermotolerance (PMID:37386471, Lee et al. 2023)
  • Six strains (CF313, YR634, GV004, GV035, YR752, OV084) significantly enhance plant heat tolerance at 45C; CF313 selected for detailed follow-up
  • Includes ecological interaction (bacterially-mediated thermotolerance), engineering design details, environmental factors, and external resources with secondary ref PMID:34135464

Test plan

  • just validate kb/communities/PMI_Variovorax_Thermotolerance_Collection.yaml passes with no issues

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New community YAML for a 25-strain Variovorax collection from Populus roots
screened for Arabidopsis thermotolerance. Six strains significantly enhance
plant heat tolerance at 45C; CF313 selected for detailed follow-up.

Primary ref: PMID:37386471 (Lee et al. 2023, Plant Methods)

Co-Authored-By: Claude Opus 4.6 <noreply@anthropic.com>
Copilot AI review requested due to automatic review settings March 5, 2026 15:04
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Pull request overview

This PR adds a new community YAML file for a PMI (Plant-Microbe Interfaces) Variovorax thermotolerance screening collection based on Lee et al. 2023 (PMID:37386471). The file describes a 25-strain Variovorax panel from Populus roots that was screened for bacterial effects on Arabidopsis thermotolerance, with six strains showing significant enhancement of plant heat tolerance at 45°C.

Changes:

  • New community YAML file PMI_Variovorax_Thermotolerance_Collection.yaml with taxonomy entries for 6 significant thermotolerance-enhancing strains, an ecological interaction (bacterially-mediated thermotolerance), environmental factors, and two external resource references (primary and secondary publications).
  • Includes properly structured schema-conformant entries with evidence items linking claims to PMID:37386471.

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Comment on lines +1 to +8
name: PMI Variovorax Thermotolerance Collection
description: >-
A 25-strain Variovorax collection from Populus roots, screened for bacterial effects
on Arabidopsis thaliana thermotolerance using a rapid hydroponic assay. Six strains
(CF313, YR634, GV004, GV035, YR752, OV084) significantly improve plant heat tolerance
at 45C. CF313 was selected for detailed follow-up study. The thermal benefit requires
direct contact with living bacteria; heat-killed bacteria and culture supernatants
provide no protection.
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The description says this is "A 25-strain Variovorax collection" and the name says "PMI Variovorax Thermotolerance Collection", but the taxonomy section only lists the 6 strains that showed significant thermotolerance. This creates an inconsistency — the file describes a 25-strain collection but only enumerates 6 members. Consider either: (1) listing all 25 strains in the taxonomy (even without individual evidence for the non-significant ones), or (2) updating the description and name to clarify this represents only the 6 thermotolerance-positive subset, e.g. "A 6-strain Variovorax subset from a 25-strain Populus root screen…".

Suggested change
name: PMI Variovorax Thermotolerance Collection
description: >-
A 25-strain Variovorax collection from Populus roots, screened for bacterial effects
on Arabidopsis thaliana thermotolerance using a rapid hydroponic assay. Six strains
(CF313, YR634, GV004, GV035, YR752, OV084) significantly improve plant heat tolerance
at 45C. CF313 was selected for detailed follow-up study. The thermal benefit requires
direct contact with living bacteria; heat-killed bacteria and culture supernatants
provide no protection.
name: PMI Variovorax Thermotolerance-Positive Subset
description: >-
A 6-strain Variovorax subset from a 25-strain Populus root collection, screened for
bacterial effects on Arabidopsis thaliana thermotolerance using a rapid hydroponic
assay. Six strains (CF313, YR634, GV004, GV035, YR752, OV084) significantly improve
plant heat tolerance at 45C. CF313 was selected for detailed follow-up study. The
thermal benefit requires direct contact with living bacteria; heat-killed bacteria
and culture supernatants provide no protection.

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Comment on lines +201 to +203
method was used to screen 25 strains of the plant growth promoting Variovorax
spp. for enhanced plant thermotolerance
explanation: The assay screens for thermotolerance under controlled heat shock conditions.
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The evidence snippet for the heat shock temperature environmental factor ("method was used to screen 25 strains of the plant growth promoting Variovorax spp. for enhanced plant thermotolerance") is a generic statement about thermotolerance screening. It does not mention the specific temperature (45°C), duration (14 minutes), or recovery conditions (4-day recovery at 22°C) claimed in the description and value fields. Per the project convention ("Evidence on everything" — CLAUDE.md line 48), snippets should directly support the specific claims. Consider finding a snippet from the paper that mentions the actual heat shock parameters.

Suggested change
method was used to screen 25 strains of the plant growth promoting Variovorax
spp. for enhanced plant thermotolerance
explanation: The assay screens for thermotolerance under controlled heat shock conditions.
Seedlings were subjected to a heat shock at 45 °C for 14 min and then returned
to 22 °C for 4 d of recovery before thermotolerance was scored.
explanation: Directly documents the heat shock temperature, duration, and recovery conditions used in the assay.

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@realmarcin realmarcin merged commit 297f39b into main Mar 6, 2026
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@realmarcin realmarcin deleted the add-pmi-variovorax-thermotolerance branch March 6, 2026 09:11
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3 participants