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Dear all,
I have a problem with my gating strategy. Probably it's just because I don't know the proper syntax.
I have done the following with my flowSet "fset":
gs=GatingSet(fset)
rg <- rectangleGate("140Ce"=c(0,10), "DNA1"=c(7, 15), filterId = "Nobead")
gs_pop_add(gs, rg, parent = "root")
recompute(gs)
I can visualize my data after the rectangular gate and see how the flowclust gate behave:
ex <- Subset(fset[[1]], rg)
dna1_and_2gate <- gate_flowclust_2d(ex, "DNA1", "DNA2", filterId = "singlet", K = 1, quantile = 0.99)
ggcyto(ex, aes(x = DNA1, y = DNA2)) + geom_hex(bins = 64) + geom_gate(dna1_and_2gate) + geom_stats()
But how can I add the gate_flowclust_2d to gs_pop_add after the rectangular gate?
Is that even possible? Also, it should be specific for every flow frame.
Thank you for your time.
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