Skip to content

WARNING closely-related and very-long sequences #7

@ireneortega

Description

@ireneortega

I run the command:
FastTree -nt -gtr core_gene_alignment.aln > core_genome_aln.newick
and printed the following warnings:

FastTree Version 2.1.11 Double precision (No SSE3)
Alignment: core_gene_alignment.aln
Nucleotide distances: Jukes-Cantor Joins: balanced Support: SH-like 1000
Search: Normal +NNI +SPR (2 rounds range 10) +ML-NNI opt-each=1
TopHits: 1.00*sqrtN close=default refresh=0.80
ML Model: Generalized Time-Reversible, CAT approximation with 20 rate categories
Initial topology in 94.44 seconds of    119    1 of    122 seqs    100)   
Refining topology: 28 rounds ME-NNIs, 2 rounds ME-SPRs, 14 rounds ML-NNIs
Total branch-length 0.116 after 925.62 sec 101 of 120 splits, 0 changes   x delta 0.000)    

WARNING! This alignment consists of closely-related and very-long sequences.
WARNING! FastTree (or other standard maximum-likelihood tools)
may not be appropriate for aligments of very closely-related sequences
like this one, as FastTree does not account for recombination or gene conversion
...

Should I be worried that the tree is not correct? How can I solve them within FastTree? Thanks!

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Projects

    No projects

    Milestone

    No milestone

    Relationships

    None yet

    Development

    No branches or pull requests

    Issue actions