tmn-basic.owl

<?xml version="1.0"?>
<rdf:RDF xmlns="http://purl.obolibrary.org/obo/tmn/tmn-basic.owl#"
     xml:base="http://purl.obolibrary.org/obo/tmn/tmn-basic.owl"
     xmlns:dc="http://purl.org/dc/elements/1.1/"
     xmlns:obo="http://purl.obolibrary.org/obo/"
     xmlns:owl="http://www.w3.org/2002/07/owl#"
     xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
     xmlns:xml="http://www.w3.org/XML/1998/namespace"
     xmlns:xsd="http://www.w3.org/2001/XMLSchema#"
     xmlns:rdfs="http://www.w3.org/2000/01/rdf-schema#"
     xmlns:terms="http://purl.org/dc/terms/"
     xmlns:oboInOwl="http://www.geneontology.org/formats/oboInOwl#">
    <owl:Ontology rdf:about="http://purl.obolibrary.org/obo/tmn/tmn-basic.owl">
        <owl:versionIRI rdf:resource="http://purl.obolibrary.org/obo/tmn/releases/2022-12-01/tmn-basic.owl"/>
        <dc:description>This is an ontology that captures techniques and methods in neuroscience to support work at AIBS and BICCN. The techniques and methods ontology is designed to help scientists communicate about their experiments and results by defining a set of terms for techniques, methods, modalities, assays, devices, tools, and the like.</dc:description>
        <dc:title>Techniques and Methods for Neuroscience Ontology</dc:title>
        <terms:license rdf:resource="https://creativecommons.org/licenses/by/4.0/"/>
        <owl:versionInfo rdf:datatype="http://www.w3.org/2001/XMLSchema#string">2022-12-01</owl:versionInfo>
    </owl:Ontology>
    


    <!-- 
    ///////////////////////////////////////////////////////////////////////////////////////
    //
    // Annotation properties
    //
    ///////////////////////////////////////////////////////////////////////////////////////
     -->

    


    <!-- http://purl.obolibrary.org/obo/IAO_0000111 -->

    <owl:AnnotationProperty rdf:about="http://purl.obolibrary.org/obo/IAO_0000111">
        <obo:IAO_0000111 xml:lang="en">editor preferred term</obo:IAO_0000111>
        <obo:IAO_0000114 rdf:resource="http://purl.obolibrary.org/obo/IAO_0000122"/>
        <obo:IAO_0000115 xml:lang="en">The concise, meaningful, and human-friendly name for a class or property preferred by the ontology developers. (US-English)</obo:IAO_0000115>
        <obo:IAO_0000117 xml:lang="en">PERSON:Daniel Schober</obo:IAO_0000117>
        <obo:IAO_0000119 xml:lang="en">GROUP:OBI:&lt;http://purl.obolibrary.org/obo/obi&gt;</obo:IAO_0000119>
        <rdfs:isDefinedBy rdf:resource="http://purl.obolibrary.org/obo/iao.owl"/>
        <rdfs:label xml:lang="en">editor preferred term</rdfs:label>
    </owl:AnnotationProperty>
    


    <!-- http://purl.obolibrary.org/obo/IAO_0000114 -->

    <owl:AnnotationProperty rdf:about="http://purl.obolibrary.org/obo/IAO_0000114"/>
    


    <!-- http://purl.obolibrary.org/obo/IAO_0000115 -->

    <owl:AnnotationProperty rdf:about="http://purl.obolibrary.org/obo/IAO_0000115">
        <obo:IAO_0000111 xml:lang="en">definition</obo:IAO_0000111>
        <obo:IAO_0000114 rdf:resource="http://purl.obolibrary.org/obo/IAO_0000122"/>
        <obo:IAO_0000115 xml:lang="en">The official definition, explaining the meaning of a class or property. Shall be Aristotelian, formalized and normalized. Can be augmented with colloquial definitions.</obo:IAO_0000115>
        <obo:IAO_0000116 xml:lang="en">2012-04-05: 
Barry Smith

The official OBI definition, explaining the meaning of a class or property: &apos;Shall be Aristotelian, formalized and normalized. Can be augmented with colloquial definitions&apos;  is terrible.

Can you fix to something like:

A statement of necessary and sufficient conditions explaining the meaning of an expression referring to a class or property.

Alan Ruttenberg

Your proposed definition is a reasonable candidate, except that it is very common that necessary and sufficient conditions are not given. Mostly they are necessary, occasionally they are necessary and sufficient or just sufficient. Often they use terms that are not themselves defined and so they effectively can&apos;t be evaluated by those criteria. 

On the specifics of the proposed definition:

We don&apos;t have definitions of &apos;meaning&apos; or &apos;expression&apos; or &apos;property&apos;. For &apos;reference&apos; in the intended sense I think we use the term &apos;denotation&apos;. For &apos;expression&apos;, I think we you mean symbol, or identifier. For &apos;meaning&apos; it differs for class and property. For class we want documentation that let&apos;s the intended reader determine whether an entity is instance of the class, or not. For property we want documentation that let&apos;s the intended reader determine, given a pair of potential relata, whether the assertion that the relation holds is true. The &apos;intended reader&apos; part suggests that we also specify who, we expect, would be able to understand the definition, and also generalizes over human and computer reader to include textual and logical definition. 

Personally, I am more comfortable weakening definition to documentation, with instructions as to what is desirable. 

We also have the outstanding issue of how to aim different definitions to different audiences. A clinical audience reading chebi wants a different sort of definition documentation/definition from a chemistry trained audience, and similarly there is a need for a definition that is adequate for an ontologist to work with.  </obo:IAO_0000116>
        <obo:IAO_0000117 xml:lang="en">PERSON:Daniel Schober</obo:IAO_0000117>
        <obo:IAO_0000119 xml:lang="en">GROUP:OBI:&lt;http://purl.obolibrary.org/obo/obi&gt;</obo:IAO_0000119>
        <rdfs:isDefinedBy rdf:resource="http://purl.obolibrary.org/obo/iao.owl"/>
        <rdfs:label>definition</rdfs:label>
        <rdfs:label xml:lang="en">definition</rdfs:label>
    </owl:AnnotationProperty>
    


    <!-- http://purl.obolibrary.org/obo/IAO_0000116 -->

    <owl:AnnotationProperty rdf:about="http://purl.obolibrary.org/obo/IAO_0000116">
        <obo:IAO_0000111 xml:lang="en">editor note</obo:IAO_0000111>
        <obo:IAO_0000114 rdf:resource="http://purl.obolibrary.org/obo/IAO_0000122"/>
        <obo:IAO_0000115 xml:lang="en">An administrative note intended for its editor. It may not be included in the publication version of the ontology, so it should contain nothing necessary for end users to understand the ontology.</obo:IAO_0000115>
        <obo:IAO_0000117 xml:lang="en">PERSON:Daniel Schober</obo:IAO_0000117>
        <obo:IAO_0000119 xml:lang="en">GROUP:OBI:&lt;http://purl.obofoundry.org/obo/obi&gt;</obo:IAO_0000119>
        <oboInOwl:hasDbXref>IAO:0000116</oboInOwl:hasDbXref>
        <oboInOwl:hasOBONamespace>uberon</oboInOwl:hasOBONamespace>
        <oboInOwl:id>editor_note</oboInOwl:id>
        <oboInOwl:is_metadata_tag rdf:datatype="http://www.w3.org/2001/XMLSchema#boolean">true</oboInOwl:is_metadata_tag>
        <oboInOwl:shorthand>editor_note</oboInOwl:shorthand>
        <rdfs:isDefinedBy rdf:resource="http://purl.obolibrary.org/obo/iao.owl"/>
        <rdfs:label>editor note</rdfs:label>
        <rdfs:label xml:lang="en">editor note</rdfs:label>
    </owl:AnnotationProperty>
    


    <!-- http://purl.obolibrary.org/obo/IAO_0000117 -->

    <owl:AnnotationProperty rdf:about="http://purl.obolibrary.org/obo/IAO_0000117">
        <obo:IAO_0000111 xml:lang="en">term editor</obo:IAO_0000111>
        <obo:IAO_0000114 rdf:resource="http://purl.obolibrary.org/obo/IAO_0000122"/>
        <obo:IAO_0000115 xml:lang="en">Name of editor entering the term in the file. The term editor is a point of contact for information regarding the term. The term editor may be, but is not always, the author of the definition, which may have been worked upon by several people</obo:IAO_0000115>
        <obo:IAO_0000116 xml:lang="en">20110707, MC: label update to term editor and definition modified accordingly. See https://github.com/information-artifact-ontology/IAO/issues/115.</obo:IAO_0000116>
        <obo:IAO_0000117 xml:lang="en">PERSON:Daniel Schober</obo:IAO_0000117>
        <obo:IAO_0000119 xml:lang="en">GROUP:OBI:&lt;http://purl.obolibrary.org/obo/obi&gt;</obo:IAO_0000119>
        <rdfs:isDefinedBy rdf:resource="http://purl.obolibrary.org/obo/iao.owl"/>
        <rdfs:label xml:lang="en">term editor</rdfs:label>
    </owl:AnnotationProperty>
    


    <!-- http://purl.obolibrary.org/obo/IAO_0000118 -->

    <owl:AnnotationProperty rdf:about="http://purl.obolibrary.org/obo/IAO_0000118">
        <obo:IAO_0000111 xml:lang="en">alternative label</obo:IAO_0000111>
        <obo:IAO_0000114 rdf:resource="http://purl.obolibrary.org/obo/IAO_0000125"/>
        <obo:IAO_0000115 xml:lang="en">A label for a class or property that can be used to refer to the class or property instead of the preferred rdfs:label. Alternative labels should be used to indicate community- or context-specific labels, abbreviations, shorthand forms and the like.</obo:IAO_0000115>
        <obo:IAO_0000117>OBO Operations committee</obo:IAO_0000117>
        <obo:IAO_0000117 xml:lang="en">PERSON:Daniel Schober</obo:IAO_0000117>
        <obo:IAO_0000119 xml:lang="en">GROUP:OBI:&lt;http://purl.obolibrary.org/obo/obi&gt;</obo:IAO_0000119>
        <rdfs:comment>Consider re-defing to: An alternative name for a class or property which can mean the same thing as the preferred name (semantically equivalent, narrow, broad or related).</rdfs:comment>
        <rdfs:label xml:lang="en">alternative label</rdfs:label>
    </owl:AnnotationProperty>
    


    <!-- http://purl.obolibrary.org/obo/IAO_0000119 -->

    <owl:AnnotationProperty rdf:about="http://purl.obolibrary.org/obo/IAO_0000119">
        <obo:IAO_0000111 xml:lang="en">definition source</obo:IAO_0000111>
        <obo:IAO_0000114 rdf:resource="http://purl.obolibrary.org/obo/IAO_0000122"/>
        <obo:IAO_0000115 xml:lang="en">Formal citation, e.g. identifier in external database to indicate / attribute source(s) for the definition. Free text indicate / attribute source(s) for the definition. EXAMPLE: Author Name, URI, MeSH Term C04, PUBMED ID, Wiki uri on 31.01.2007</obo:IAO_0000115>
        <obo:IAO_0000117 xml:lang="en">PERSON:Daniel Schober</obo:IAO_0000117>
        <obo:IAO_0000119 xml:lang="en">Discussion on obo-discuss mailing-list, see http://bit.ly/hgm99w</obo:IAO_0000119>
        <obo:IAO_0000119 xml:lang="en">GROUP:OBI:&lt;http://purl.obolibrary.org/obo/obi&gt;</obo:IAO_0000119>
        <rdfs:isDefinedBy rdf:resource="http://purl.obolibrary.org/obo/iao.owl"/>
        <rdfs:label xml:lang="en">definition source</rdfs:label>
    </owl:AnnotationProperty>
    


    <!-- http://purl.obolibrary.org/obo/IAO_0000232 -->

    <owl:AnnotationProperty rdf:about="http://purl.obolibrary.org/obo/IAO_0000232">
        <obo:IAO_0000111 xml:lang="en">curator note</obo:IAO_0000111>
        <obo:IAO_0000114 rdf:resource="http://purl.obolibrary.org/obo/IAO_0000122"/>
        <obo:IAO_0000115 xml:lang="en">An administrative note of use for a curator but of no use for a user</obo:IAO_0000115>
        <obo:IAO_0000117 xml:lang="en">PERSON:Alan Ruttenberg</obo:IAO_0000117>
        <oboInOwl:hasDbXref>IAO:0000232</oboInOwl:hasDbXref>
        <oboInOwl:hasOBONamespace>uberon</oboInOwl:hasOBONamespace>
        <oboInOwl:id>curator_notes</oboInOwl:id>
        <oboInOwl:is_metadata_tag rdf:datatype="http://www.w3.org/2001/XMLSchema#boolean">true</oboInOwl:is_metadata_tag>
        <oboInOwl:shorthand>curator_notes</oboInOwl:shorthand>
        <rdfs:isDefinedBy rdf:resource="http://purl.obolibrary.org/obo/iao.owl"/>
        <rdfs:label xml:lang="en">curator note</rdfs:label>
        <rdfs:label>curator notes</rdfs:label>
    </owl:AnnotationProperty>
    


    <!-- http://purl.org/dc/elements/1.1/description -->

    <owl:AnnotationProperty rdf:about="http://purl.org/dc/elements/1.1/description">
        <oboInOwl:hasDbXref>http://purl.org/dc/elements/1.1/description</oboInOwl:hasDbXref>
        <oboInOwl:hasOBONamespace>uberon</oboInOwl:hasOBONamespace>
        <oboInOwl:id>dc-description</oboInOwl:id>
        <oboInOwl:is_metadata_tag rdf:datatype="http://www.w3.org/2001/XMLSchema#boolean">true</oboInOwl:is_metadata_tag>
        <oboInOwl:shorthand>dc-description</oboInOwl:shorthand>
        <rdfs:label>description</rdfs:label>
    </owl:AnnotationProperty>
    


    <!-- http://purl.org/dc/elements/1.1/title -->

    <owl:AnnotationProperty rdf:about="http://purl.org/dc/elements/1.1/title">
        <oboInOwl:hasDbXref>http://purl.org/dc/elements/1.1/title</oboInOwl:hasDbXref>
        <oboInOwl:hasOBONamespace>uberon</oboInOwl:hasOBONamespace>
        <oboInOwl:id>dc-title</oboInOwl:id>
        <oboInOwl:is_metadata_tag rdf:datatype="http://www.w3.org/2001/XMLSchema#boolean">true</oboInOwl:is_metadata_tag>
        <oboInOwl:shorthand>dc-title</oboInOwl:shorthand>
        <rdfs:label>title</rdfs:label>
    </owl:AnnotationProperty>
    


    <!-- http://purl.org/dc/terms/license -->

    <owl:AnnotationProperty rdf:about="http://purl.org/dc/terms/license">
        <oboInOwl:hasDbXref>http://purl.org/dc/terms/license</oboInOwl:hasDbXref>
        <oboInOwl:hasOBONamespace>uberon</oboInOwl:hasOBONamespace>
        <oboInOwl:id>dcterms-license</oboInOwl:id>
        <oboInOwl:is_metadata_tag rdf:datatype="http://www.w3.org/2001/XMLSchema#boolean">true</oboInOwl:is_metadata_tag>
        <oboInOwl:shorthand>dcterms-license</oboInOwl:shorthand>
        <rdfs:label>license</rdfs:label>
    </owl:AnnotationProperty>
    


    <!-- http://purl.org/dc/terms/source -->

    <owl:AnnotationProperty rdf:about="http://purl.org/dc/terms/source"/>
    


    <!-- http://www.geneontology.org/formats/oboInOwl#SubsetProperty -->

    <owl:AnnotationProperty rdf:about="http://www.geneontology.org/formats/oboInOwl#SubsetProperty"/>
    


    <!-- http://www.geneontology.org/formats/oboInOwl#SynonymTypeProperty -->

    <owl:AnnotationProperty rdf:about="http://www.geneontology.org/formats/oboInOwl#SynonymTypeProperty"/>
    


    <!-- http://www.geneontology.org/formats/oboInOwl#created_by -->

    <owl:AnnotationProperty rdf:about="http://www.geneontology.org/formats/oboInOwl#created_by"/>
    


    <!-- http://www.geneontology.org/formats/oboInOwl#creation_date -->

    <owl:AnnotationProperty rdf:about="http://www.geneontology.org/formats/oboInOwl#creation_date"/>
    


    <!-- http://www.geneontology.org/formats/oboInOwl#hasAlternativeId -->

    <owl:AnnotationProperty rdf:about="http://www.geneontology.org/formats/oboInOwl#hasAlternativeId">
        <rdfs:label>has_alternative_id</rdfs:label>
    </owl:AnnotationProperty>
    


    <!-- http://www.geneontology.org/formats/oboInOwl#hasBroadSynonym -->

    <owl:AnnotationProperty rdf:about="http://www.geneontology.org/formats/oboInOwl#hasBroadSynonym">
        <rdfs:label>has_broad_synonym</rdfs:label>
    </owl:AnnotationProperty>
    


    <!-- http://www.geneontology.org/formats/oboInOwl#hasDbXref -->

    <owl:AnnotationProperty rdf:about="http://www.geneontology.org/formats/oboInOwl#hasDbXref"/>
    


    <!-- http://www.geneontology.org/formats/oboInOwl#hasExactSynonym -->

    <owl:AnnotationProperty rdf:about="http://www.geneontology.org/formats/oboInOwl#hasExactSynonym">
        <rdfs:label>has_exact_synonym</rdfs:label>
    </owl:AnnotationProperty>
    


    <!-- http://www.geneontology.org/formats/oboInOwl#hasOBONamespace -->

    <owl:AnnotationProperty rdf:about="http://www.geneontology.org/formats/oboInOwl#hasOBONamespace"/>
    


    <!-- http://www.geneontology.org/formats/oboInOwl#id -->

    <owl:AnnotationProperty rdf:about="http://www.geneontology.org/formats/oboInOwl#id"/>
    


    <!-- http://www.geneontology.org/formats/oboInOwl#is_metadata_tag -->

    <owl:AnnotationProperty rdf:about="http://www.geneontology.org/formats/oboInOwl#is_metadata_tag"/>
    


    <!-- http://www.geneontology.org/formats/oboInOwl#shorthand -->

    <owl:AnnotationProperty rdf:about="http://www.geneontology.org/formats/oboInOwl#shorthand"/>
    


    <!-- 
    ///////////////////////////////////////////////////////////////////////////////////////
    //
    // Classes
    //
    ///////////////////////////////////////////////////////////////////////////////////////
     -->

    


    <!-- http://purl.obolibrary.org/obo/TMN_0000000 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000000">
        <obo:IAO_0000115 xml:lang="en">A sequencing assay that analyzes mRNA transcripts from droplets of individual cells. The droplets are compartmentalized via a microfluidic device. After the cells are compartmentalized, cells in droplets are lysed and the released mRNA hybridizes to the oligo(dT) tract of the primer beads. Then the droplets are pooled and broken to release the beads which are then isolated and reverse-transcribed with template switching. The resulting cDNA strands are PCR-amplified and the sequencing adapters are added. The barcoded mRNA samples are then sequenced.</obo:IAO_0000115>
        <obo:IAO_0000117 xml:lang="en">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000118 xml:lang="en">Dropseq</obo:IAO_0000118>
        <obo:IAO_0000119 xml:lang="en">https://doi.org/10.1016/j.cell.2015.05.002</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-03T20:04:39Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">droplet-based single-cell RNA sequencing assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000001 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000001">
        <obo:IAO_0000115 xml:lang="en">A sequencing assay that uses fluorescent probes that bind to parts of a nucleic acid sequence that have a high degree of sequence complementarity. Fluorescence microscopy can be used to find out where the fluorescent probes are bound on the chromosomes.</obo:IAO_0000115>
        <obo:IAO_0000117 xml:lang="en">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000118 xml:lang="en">FISH</obo:IAO_0000118>
        <obo:IAO_0000119 xml:lang="en">http://en.wikipedia.org/wiki/Fluorescence_in_situ_hybridization</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-03T21:04:00Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">fluorescence in situ hybridization assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000002 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000002">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">An imaging assay that uses micro-optical sectioning tomography technology combined with fluorescence molecular labeling of neuronal projections.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">fMOST</obo:IAO_0000118>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6701528/</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-03T21:32:43Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">fluorescence micro-optical sectioning tomography assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000003 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000003">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">An imaging assay that measures the copy number and spatial distribution of hundreds to thousands of RNA species in single cells.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">MERFISH</obo:IAO_0000118>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">DOI: 10.1126/science.aaa6090</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-03T23:18:34Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">multiplexed error-robust fluorescence in situ hybridization assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000004 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000004">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A 3D structure determination assay that determines the morphology of a neuron.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">3D structure determination assay of neuron</obo:IAO_0000118>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-04T00:06:14Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">neuron morphology assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000005 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000005">
        <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/TMN_0000004"/>
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A neuron morphology assay that enables sparse and stochastic labeling of genetically-defined neurons. MORF-labeled neurons are digitally reconstructed using a program (G-Cut).</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">https://biccn.org/teams/u01-yang</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-04T00:10:17Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">mosaicism with repeat frameshift genetic sparse labeling assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000006 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000006">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A fluorescence microscopy that generates high-throughput flourescence imaging of mouse brains by integrating two-photon microscopy and tissue sectioning. Serial two-photon tomography generates high-resolution datasets.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">STPT</obo:IAO_0000118>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://doi.org/10.1038/nmeth.1854</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-04T00:30:35Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">serial two-photon tomography</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000007 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000007">
        <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/TMN_0000006"/>
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A serial two photon tomography assay that produces images of mouse brains with sufficient detail to represent axons of individual neurons.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://www.janelia.org/project-team/mouselight/research</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-04T00:34:49Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">mouselight</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000008 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000008">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">An electrophysiology assay that employs many electrodes to record extracellular field potentials.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://en.wikipedia.org/wiki/Microelectrode_array</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-10T23:56:39Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">multi-electrode extracellular electrophysiology assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000009 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000009">
        <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/TMN_0000004"/>
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A neuron morphology assay where the morphology or structure of a neuron is represented through through another medium. Often, this assay is a combination of different assays (sparse labeling, fluorescent labeling, fMOST).</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://doi.org/10.1101/675280</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-11T00:15:55Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">neuron morphology reconstruction assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000010 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000010">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A single-cell RNA sequencing assay wherein a patch clamp recording is taken from a neuron and then scRNA sequencing is performed on the cell soma.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patch clamp sequencing assay</obo:IAO_0000118>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patch-seq</obo:IAO_0000118>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://doi.org/10.1038/nprot.2017.120</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-11T00:26:12Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">patch clamp single-cell RNA sequencing assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000011 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000011">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">An assay wherein a substance is taken up by an axon terminal and transported toward the soma to mark (or trace) some part of a cell.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">retrograde tracing</obo:IAO_0000118>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-11T00:39:16Z</oboInOwl:creation_date>
        <oboInOwl:hasExactSynonym xml:lang="en">retrograde labeling</oboInOwl:hasExactSynonym>
        <rdfs:label xml:lang="en">retrograde tracing assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000012 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000012">
        <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/TMN_0000011"/>
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A retrograde tracing assay that traces the neural connections from a synapse to a soma allowing the mapping of connections between neurons in a structure and a target in the brain.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://en.wikipedia.org/wiki/Retrograde_tracing</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-11T00:51:53Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">retrograde transsynaptic tracing assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000013 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000013">
        <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/TMN_0000001"/>
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A fluorescence in situ hybridization assay where transcripts are labeled with fluorescent probes in sequential rounds of hybridization to read out the temporal barcode for each transcript. The temporal barcodes can then be decoded to uniquely identify the RNA transcripts present in the sample. RNA can be identified directly in a variety of samples from extracted RNA to tissues.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">seqFISH</obo:IAO_0000118>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://www.seqfish.com/technology</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-11T01:00:00Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">sequential fluorescence in situ hybridization assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000014 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000014">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A single cell sequencing assay wherein the target cells are lysed, and the RNA is hybridized to an oligo(dT)-containing primer. The first strand of the cDNA is synthesized with the addition of a few untemplated C nucleotides. This poly(C) overhang is added exclusively to full-length transcripts. An oligonucleotide primer is hybridized to the poly(C) overhang and used to synthesize the second strand. Full-length cDNAs are PCR-amplified to obtain nanogram amounts of DNA. The PCR products are purified for sequencing.</obo:IAO_0000115>
        <obo:IAO_0000116 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Definition needs curation.</obo:IAO_0000116>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">SMART seq</obo:IAO_0000118>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">SMARTseq</obo:IAO_0000118>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://www.enseqlopedia.com/wiki-entry/rna-sequencing-methods/low-level-rna-detection/smart-seq/</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-11T01:10:58Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">switching mechanism at the 5&apos; end of RNA template sequencing assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000015 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000015">
        <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/TMN_0000024"/>
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A switching mechanism at the 5&apos; end of RNA template sequencing assay that is the fourth version of such an assay.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">SMARTseq v4</obo:IAO_0000118>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000119>
        <obo:IAO_0000232 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">This is the commercial version of SMARTseq2 that has as an instrument the Takara kit.</obo:IAO_0000232>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-11T01:21:33Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">switching mechanism at the 5&apos; end of RNA template sequencing assay v4</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000016 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000016">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A study design in which complementary viruses are to be injected into brain regions to monosynaptically label inputs to a specific projection neuron population.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">TRIO tracing</obo:IAO_0000118>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://cic.ini.usc.edu/protocols/trio-tracing</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-11T01:43:24Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">tracing the relationship between input and output study design</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000017 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000017">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A fluorescence microscopy assay that uses a laser light-sheet to illuminate a region of a sample that has been treated to excite fluorescently.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">light sheet microscopy</obo:IAO_0000118>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://en.wikipedia.org/wiki/Light_sheet_fluorescence_microscopy</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-11T01:59:49Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">light sheet fluorescence microscopy assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000018 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000018">
        <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/TMN_0000019"/>
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A anterograde tracing assay that uses a Cre recombinase-dependent as a tracer.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://doi.org/10.1016/j.neuron.2011.12.002</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-15T18:11:42Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">cre-dependent anterograde tracing assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000019 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000019">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">An assay wherein axon projections are traced from their source (cell body or soma) to their point of termination (synapse).</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">https://en.wikipedia.org/wiki/Anterograde_tracing</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-15T18:13:29Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">anterograde tracing assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000020 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000020">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">An imaging assay that has as an output label-free, cross-sectional imaging of biological tissue microstructure using tissue backscattering properties.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OCT</obo:IAO_0000118>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PMID: 29214158</obo:IAO_0000119>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">doi: 10.1155/2017/3409327</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-15T18:44:49Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">optical coherence tomography assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000021 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000021">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A light sheet microscopy assay that combines synchronized scanning illumination and oblique imaging over cleared, thick tissue sections in smooth motion that produces a 3D image of a brain.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">VISoR</obo:IAO_0000118>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">VISoR assay</obo:IAO_0000118>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">doi: 10.1093/nsr/nwz053</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-15T19:01:49Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">volumetric imaging with synchronized on-the-fly oblique-scan and readout assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000022 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000022">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A single-cell RNA sequencing assay that involves the isolation of single nuclei and open chromatin tagmentation in the isolated nuclei using a transposase. The tagmented nuclei are then encapsulated in a droplet including both an oligo-dT-containing barcoded bead and a splint oligonucleotide, which links the tagmented gDNA fragments to the bead, enabling the bead to capture both mRNAs and open chromatin fragments. After mRNAs and gDNA fragments are released from the bead by heating, RT and PCR amplification are performed to generate a library of cDNA and open chromatin gDNA fragments.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">SNARE-seq</obo:IAO_0000118>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">SNARE-seq assay</obo:IAO_0000118>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">https://doi.org/10.1038/s12276-020-0420-2</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-15T19:09:43Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">single-nucleus chromatin accessibility and mRNA expression sequencing assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000023 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000023">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A fluorescence microscopy assay wherein fluorescence in situ hybridization is leveraged with multiplexed analysis that uses each channel to measure the composite (sum) abundances of multiple genes in each channel and decompresses and determines individual gene level.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">CISI</obo:IAO_0000118>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">DOI: 10.1101/743039</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-02-15T19:20:54Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">composite in situ imaging</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000024 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000024">
        <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/TMN_0000014"/>
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A switching mechanism at the 5&apos; end of RNA template sequencing assay that allows the generation of full-length cDNA and sequencing libraries by using standard reagents.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">SMARTseq2</obo:IAO_0000118>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">https://doi.org/10.1038/nprot.2014.006</obo:IAO_0000119>
        <obo:IAO_0000232 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">See also: https://scicrunch.org/scicrunch/interlex/view/ilx_0739685</obo:IAO_0000232>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-04-22T19:49:20Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">switching mechanism at the 5&apos; end of RNA template sequencing assay 2</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000025 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000025">
        <obo:IAO_0000115 xml:lang="en">A RNA sequencing assay that involves three levels of indexing.</obo:IAO_0000115>
        <obo:IAO_0000118 xml:lang="en">3-level sci-RNA-seq</obo:IAO_0000118>
        <obo:IAO_0000118 xml:lang="en">3-level single-cell combinatorial indexing RNA sequencing assay</obo:IAO_0000118>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-09-02T02:12:07Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">sci-RNA-seq3</rdfs:label>
    </owl:Class>
    <owl:Axiom>
        <owl:annotatedSource rdf:resource="http://purl.obolibrary.org/obo/TMN_0000025"/>
        <owl:annotatedProperty rdf:resource="http://purl.obolibrary.org/obo/IAO_0000115"/>
        <owl:annotatedTarget xml:lang="en">A RNA sequencing assay that involves three levels of indexing.</owl:annotatedTarget>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">https://www.protocols.io/view/sci-rna-seq3-9yih7ue</obo:IAO_0000119>
    </owl:Axiom>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000026 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000026">
        <obo:IAO_0000115 xml:lang="en">An ATAC-seq assay that involves single-cell combinatorial indexing at three levels.</obo:IAO_0000115>
        <obo:IAO_0000118 xml:lang="en">3-level sci-ATAC-seq</obo:IAO_0000118>
        <obo:IAO_0000118 xml:lang="en">3-level single-cell combinatorial indexing ATAC sequencing assay</obo:IAO_0000118>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-09-02T02:16:18Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">sci-ATAC-seq3</rdfs:label>
    </owl:Class>
    <owl:Axiom>
        <owl:annotatedSource rdf:resource="http://purl.obolibrary.org/obo/TMN_0000026"/>
        <owl:annotatedProperty rdf:resource="http://purl.obolibrary.org/obo/IAO_0000115"/>
        <owl:annotatedTarget xml:lang="en">An ATAC-seq assay that involves single-cell combinatorial indexing at three levels.</owl:annotatedTarget>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">https://www.protocols.io/view/sci-atac-seq3-be8mjhu6</obo:IAO_0000119>
    </owl:Axiom>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000027 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000027">
        <obo:IAO_0000115 xml:lang="en">An ATAC-seq assay that involves use of the 10x sequencing technology assay for transposase-accessible chromatin using sequencing (ATAC-seq).</obo:IAO_0000115>
        <obo:IAO_0000118 xml:lang="en">10x chromium ATAC sequencing assay</obo:IAO_0000118>
        <obo:IAO_0000118 xml:lang="en">10x chromium ATAC-seq assay</obo:IAO_0000118>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-09-02T02:18:54Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">10x chromium ATAC-seq</rdfs:label>
    </owl:Class>
    <owl:Axiom>
        <owl:annotatedSource rdf:resource="http://purl.obolibrary.org/obo/TMN_0000027"/>
        <owl:annotatedProperty rdf:resource="http://purl.obolibrary.org/obo/IAO_0000115"/>
        <owl:annotatedTarget xml:lang="en">An ATAC-seq assay that involves use of the 10x sequencing technology assay for transposase-accessible chromatin using sequencing (ATAC-seq).</owl:annotatedTarget>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000119>
    </owl:Axiom>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000028 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000028">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A RNA-seq assay for transferring RNA from tissue sections onto a surface covered in DNA-barcoded beads with known positions, allowing the locations of the RNA to be inferred by sequencing.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">slide-seq</obo:IAO_0000118>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">slide-sequencing assay</obo:IAO_0000118>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000119>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">doi: 10.1126/science.aaw1219</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-10-04T22:03:50Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">slide-seq assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000029 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000029">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A RNA-seq assay that uses high-throughput ATAC and RNA expression with sequencing to measure chromatin accessibility and gene expression in the same single cell.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">SHARE-seq</obo:IAO_0000118>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">SHARE-sequencing</obo:IAO_0000118>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000119>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">https://doi.org/10.1016/j.cell.2020.09.056</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-10-04T22:09:37Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">SHARE-seq assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000030 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000030">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">An ATAC-seq assay that provides information about both transcriptome and epigenome from a single cell.</obo:IAO_0000115>
        <obo:IAO_0000117 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000117>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">single cell multiome ATAC + gene expression</obo:IAO_0000118>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PERSON: Patrick Ray</obo:IAO_0000119>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">https://www.10xgenomics.com/blog/introducing-chromium-single-cell-multiome-atac-gene-expression</obo:IAO_0000119>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">https://www.10xgenomics.com/products/single-cell-multiome-atac-plus-gene-expression</obo:IAO_0000119>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-10-04T22:12:23Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">single cell multiome ATAC + gene expression assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000031 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000031">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A single-cell RNA sequencing assay wherein sparse fluorescently labeled single neurons are collected manually from freshly dissociated mouse brain tissue. Then single-labeled neurons with high purity are integrated with an in vitro transcription-based amplification protocol that preserves endogenous transcript ratios. Subsequently a double linear amplification method that uses unique molecule identifiers (UMIs) is used to generate individual mRNA counts. Two rounds of amplification follow.</obo:IAO_0000115>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">DIVA-seq</obo:IAO_0000118>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-04-05T20:19:26Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">double in vitro transcription with absoluted counts sequencing assay</rdfs:label>
    </owl:Class>
    <owl:Axiom>
        <owl:annotatedSource rdf:resource="http://purl.obolibrary.org/obo/TMN_0000031"/>
        <owl:annotatedProperty rdf:resource="http://purl.obolibrary.org/obo/IAO_0000115"/>
        <owl:annotatedTarget rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A single-cell RNA sequencing assay wherein sparse fluorescently labeled single neurons are collected manually from freshly dissociated mouse brain tissue. Then single-labeled neurons with high purity are integrated with an in vitro transcription-based amplification protocol that preserves endogenous transcript ratios. Subsequently a double linear amplification method that uses unique molecule identifiers (UMIs) is used to generate individual mRNA counts. Two rounds of amplification follow.</owl:annotatedTarget>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://doi.org/10.3791/58690</obo:IAO_0000119>
    </owl:Axiom>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000032 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000032">
        <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/TMN_0000017"/>
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A light sheet tomography assay wherein the sample is imaged from the top using oblique light sheet fluorescence microscopy illumination and detection strategy and the imaged top portion of the tissue is removed by vibratome sectioning.</obo:IAO_0000115>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">OLST</obo:IAO_0000118>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-04-05T20:22:10Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">oblique light sheet tomography assay</rdfs:label>
    </owl:Class>
    <owl:Axiom>
        <owl:annotatedSource rdf:resource="http://purl.obolibrary.org/obo/TMN_0000032"/>
        <owl:annotatedProperty rdf:resource="http://purl.obolibrary.org/obo/IAO_0000115"/>
        <owl:annotatedTarget rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A light sheet tomography assay wherein the sample is imaged from the top using oblique light sheet fluorescence microscopy illumination and detection strategy and the imaged top portion of the tissue is removed by vibratome sectioning.</owl:annotatedTarget>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://doi.org/10.1101/132423</obo:IAO_0000119>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://doi.org/10.17504/protocols.io.smwec7e</obo:IAO_0000119>
    </owl:Axiom>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000033 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000033">
        <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/TMN_0000001"/>
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A cyclic single molecule fluorescent in situ hybridization assay used to quantify the expression level of specific transcripts in tissue sections by direct labeling of individual RNA molecules.</obo:IAO_0000115>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">osmFISH</obo:IAO_0000118>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-04-05T20:26:01Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">cyclic single-molecule fluorescence in situ hybridization assay</rdfs:label>
    </owl:Class>
    <owl:Axiom>
        <owl:annotatedSource rdf:resource="http://purl.obolibrary.org/obo/TMN_0000033"/>
        <owl:annotatedProperty rdf:resource="http://purl.obolibrary.org/obo/IAO_0000115"/>
        <owl:annotatedTarget rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A cyclic single molecule fluorescent in situ hybridization assay used to quantify the expression level of specific transcripts in tissue sections by direct labeling of individual RNA molecules.</owl:annotatedTarget>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://doi.org/10.1038/s41592-018-0175-z</obo:IAO_0000119>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://doi.org/10.17504/protocols.io.psednbe</obo:IAO_0000119>
    </owl:Axiom>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000034 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000034">
        <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/TMN_0000022"/>
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A single-cell RNA sequencing assay that involves the isolation of single nuclei and open chromatin tagmentation in the isolated nuclei using a transposase. The tagmented nuclei are then encapsulated in a droplet including both an oligo-dT-containing barcoded bead and a splint oligonucleotide, which links the tagmented gDNA fragments to the bead, enabling the bead to capture both mRNAs and open chromatin fragments. After mRNAs and gDNA fragments are released from the bead by heating, RT and PCR amplification are performed to generate a library of cDNA and open chromatin gDNA fragments.

This is the second version of SNARE-seq.</obo:IAO_0000115>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">SNARE-seq2</obo:IAO_0000118>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">SNARE-seq2 assay</obo:IAO_0000118>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-04-05T20:36:54Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">single-nucleus chromatin accessibility and mRNA expression sequencing assay version 2</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000035 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000035">
        <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/TMN_0000034"/>
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A SNARE-seq v2 assay that utilizes ATAC-seq as a part.</obo:IAO_0000115>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">SNARE-seq2 (ATAC-seq)</obo:IAO_0000118>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-04-05T20:43:10Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">single-nucleus chromatin accessibility and mRNA expression sequencing assay version 2 with assay for transposase-accessible chromatin using sequencing assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000036 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000036">
        <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/TMN_0000034"/>
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A SNARE-seq v2 assay that utilizes RNA-seq as a part.</obo:IAO_0000115>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">SNARE-seq2 (RNA-seq)</obo:IAO_0000118>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-04-05T20:44:55Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">single-nucleus chromatin accessibility and mRNA expression sequencing assay version 2 with RNA sequencing assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000037 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000037">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A DNA sequencing assay that provides direct, real-time analysis of long DNA or RNA fragments by monitoring changes to an electrical current as nucleic acids are passed through a protein nanopore. The resulting signal is decoded to provide the specific DNA or RNA sequence.</obo:IAO_0000115>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-05-05T17:35:14Z</oboInOwl:creation_date>
        <rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">nanopore DNA sequencing assay</rdfs:label>
    </owl:Class>
    <owl:Axiom>
        <owl:annotatedSource rdf:resource="http://purl.obolibrary.org/obo/TMN_0000037"/>
        <owl:annotatedProperty rdf:resource="http://purl.obolibrary.org/obo/IAO_0000115"/>
        <owl:annotatedTarget rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A DNA sequencing assay that provides direct, real-time analysis of long DNA or RNA fragments by monitoring changes to an electrical current as nucleic acids are passed through a protein nanopore. The resulting signal is decoded to provide the specific DNA or RNA sequence.</owl:annotatedTarget>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">https://nanoporetech.com/applications/dna-nanopore-sequencing</obo:IAO_0000119>
    </owl:Axiom>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000038 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000038">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">An isoform sequencing assay that begins with bulk tissue and identifies single-cell types and their full-length RNA isoforms without fluorescence-activated cell sorting.</obo:IAO_0000115>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">ScISOr-seq</obo:IAO_0000118>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-05-05T17:59:23Z</oboInOwl:creation_date>
        <rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">single cell isoform RNA sequencing assay</rdfs:label>
    </owl:Class>
    <owl:Axiom>
        <owl:annotatedSource rdf:resource="http://purl.obolibrary.org/obo/TMN_0000038"/>
        <owl:annotatedProperty rdf:resource="http://purl.obolibrary.org/obo/IAO_0000115"/>
        <owl:annotatedTarget rdf:datatype="http://www.w3.org/2001/XMLSchema#string">An isoform sequencing assay that begins with bulk tissue and identifies single-cell types and their full-length RNA isoforms without fluorescence-activated cell sorting.</owl:annotatedTarget>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">PMID:30320766</obo:IAO_0000119>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">https://doi.org/10.1038/nbt.4259</obo:IAO_0000119>
    </owl:Axiom>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000039 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000039">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A single-cell RNA sequencing assay that jointly profiles chromatin conformation and DNA methylation information from the same cell.</obo:IAO_0000115>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">sn-m3C-seq</obo:IAO_0000118>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-05-05T19:17:37Z</oboInOwl:creation_date>
        <rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">single-nucleus methyl-3C sequencing assay</rdfs:label>
    </owl:Class>
    <owl:Axiom>
        <owl:annotatedSource rdf:resource="http://purl.obolibrary.org/obo/TMN_0000039"/>
        <owl:annotatedProperty rdf:resource="http://purl.obolibrary.org/obo/IAO_0000115"/>
        <owl:annotatedTarget rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A single-cell RNA sequencing assay that jointly profiles chromatin conformation and DNA methylation information from the same cell.</owl:annotatedTarget>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">https://doi.org/10.1038/s41592-019-0547-z</obo:IAO_0000119>
    </owl:Axiom>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000040 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000040">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A library preparation method for single-nucleus methylome library preparation that provides significant improvements in read mapping, amount of artifactual reads, throughput, library complexity, and coverage uniformity over snmC-seq.</obo:IAO_0000115>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">snmC-seq2</obo:IAO_0000118>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-05-05T20:16:03Z</oboInOwl:creation_date>
        <rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">single-nucleus methyl-C sequencing assay version 2</rdfs:label>
    </owl:Class>
    <owl:Axiom>
        <owl:annotatedSource rdf:resource="http://purl.obolibrary.org/obo/TMN_0000040"/>
        <owl:annotatedProperty rdf:resource="http://purl.obolibrary.org/obo/IAO_0000115"/>
        <owl:annotatedTarget rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A library preparation method for single-nucleus methylome library preparation that provides significant improvements in read mapping, amount of artifactual reads, throughput, library complexity, and coverage uniformity over snmC-seq.</owl:annotatedTarget>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://doi.org/10.1038/s41467-018-06355-2</obo:IAO_0000119>
    </owl:Axiom>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000041 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000041">
        <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/TMN_0000040"/>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-05-05T21:09:57Z</oboInOwl:creation_date>
        <rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">mC-seq3</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000042 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000042">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">The second version of the single-cell combinatorial indexing with WGBS methylation analysis.</obo:IAO_0000115>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-05-05T21:34:40Z</oboInOwl:creation_date>
        <rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">single-cell combinatorial indexing with WGBS methylation analysis version 2</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000043 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000043">
        <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/TMN_0000044"/>
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">An immunoassay that uses magnetic beads to measure multiple analytes in a single assay.</obo:IAO_0000115>
        <oboInOwl:created_by rdf:datatype="http://www.w3.org/2001/XMLSchema#string">patrick-lloyd-ray</oboInOwl:created_by>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-05-05T22:19:17Z</oboInOwl:creation_date>
        <rdfs:label rdf:datatype="http://www.w3.org/2001/XMLSchema#string">multiplex immunoassay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000044 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000044">
        <obo:IAO_0000115 xml:lang="en">An assay that enables the presence or the concentration of a substance in a material to be measured by exploiting the specific binding between an analyte and the corresponding detection antibody.</obo:IAO_0000115>
        <oboInOwl:created_by rdf:resource="http://orcid.org/0000-0002-5260-9315"/>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-06-07T16:53:51Z</oboInOwl:creation_date>
        <rdfs:label xml:lang="en">immunoassay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000045 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000045">
        <obo:IAO_0000115 xml:lang="en">An imaging assay that is designed to show the calcium (Ca2+) levels of a tissue or medium.</obo:IAO_0000115>
        <oboInOwl:created_by rdf:resource="http://orcid.org/0000-0002-5260-9315"/>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-06-07T16:56:02Z</oboInOwl:creation_date>
        <oboInOwl:hasExactSynonym xml:lang="en">Ca imaging</oboInOwl:hasExactSynonym>
        <oboInOwl:hasExactSynonym xml:lang="en">Ca imaging assay</oboInOwl:hasExactSynonym>
        <oboInOwl:hasExactSynonym xml:lang="en">Ca2+ imaging</oboInOwl:hasExactSynonym>
        <oboInOwl:hasExactSynonym xml:lang="en">Ca2+ imaging assay</oboInOwl:hasExactSynonym>
        <rdfs:label xml:lang="en">calcium imaging assay</rdfs:label>
    </owl:Class>
    <owl:Axiom>
        <owl:annotatedSource rdf:resource="http://purl.obolibrary.org/obo/TMN_0000045"/>
        <owl:annotatedProperty rdf:resource="http://purl.obolibrary.org/obo/IAO_0000115"/>
        <owl:annotatedTarget xml:lang="en">An imaging assay that is designed to show the calcium (Ca2+) levels of a tissue or medium.</owl:annotatedTarget>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">http://en.wikipedia.org/wiki/Calcium_imaging</obo:IAO_0000119>
        <terms:source rdf:datatype="http://www.w3.org/2001/XMLSchema#string">ERO:0000634</terms:source>
    </owl:Axiom>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000046 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000046">
        <obo:IAO_0000115 xml:lang="en">An assay that generates data about the presence, quantity, structure, function, behavior, or activity of cells, or a process that occurs at a cellular level of anatomical granularity (includes subcellular structures and organelles).</obo:IAO_0000115>
        <oboInOwl:created_by rdf:resource="http://orcid.org/0000-0002-5260-9315"/>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-06-07T16:59:58Z</oboInOwl:creation_date>
        <oboInOwl:hasBroadSynonym xml:lang="en">cell analysis</oboInOwl:hasBroadSynonym>
        <rdfs:label xml:lang="en">cellular assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000049 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000049">
        <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/TMN_0000017"/>
        <obo:IAO_0000115 xml:lang="en">A light sheet fluorescence microscopy assay that employs high-speed scanning single-objective light sheet imaging with spectral multiplexing and cell-type encoding. Human brain samples are processed using a clearing and immunostaining approach called HuB.Clear which can generate optically clear, 5 mm thick, whole-mount brain slabs. High-field magnetic resonance imaging (MRI) data acquired on the intact, fixed brain prior to sectioning will provide a vital cross-reference for comparison while also facilitating registration of data to a common coordinate frameworks. HOLiS data will be analyzed to extract cell locations and cell type identities, generating databases that can be compared to standard neuroanatomical atlases, while also permitting careful examination of brain-wide patterns and inter-subject differences.</obo:IAO_0000115>
        <obo:IAO_0000118 xml:lang="en">HOLiS</obo:IAO_0000118>
        <oboInOwl:created_by rdf:resource="http://orcid.org/0000-0002-5260-9315"/>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-07-11T22:11:00Z</oboInOwl:creation_date>
        <rdfs:label>human brain optimized light sheet microscopy assay</rdfs:label>
    </owl:Class>
    <owl:Axiom>
        <owl:annotatedSource rdf:resource="http://purl.obolibrary.org/obo/TMN_0000049"/>
        <owl:annotatedProperty rdf:resource="http://purl.obolibrary.org/obo/IAO_0000115"/>
        <owl:annotatedTarget xml:lang="en">A light sheet fluorescence microscopy assay that employs high-speed scanning single-objective light sheet imaging with spectral multiplexing and cell-type encoding. Human brain samples are processed using a clearing and immunostaining approach called HuB.Clear which can generate optically clear, 5 mm thick, whole-mount brain slabs. High-field magnetic resonance imaging (MRI) data acquired on the intact, fixed brain prior to sectioning will provide a vital cross-reference for comparison while also facilitating registration of data to a common coordinate frameworks. HOLiS data will be analyzed to extract cell locations and cell type identities, generating databases that can be compared to standard neuroanatomical atlases, while also permitting careful examination of brain-wide patterns and inter-subject differences.</owl:annotatedTarget>
        <obo:IAO_0000119 xml:lang="en">https://www.humanbrainmapping.org/files/2022/Annual%20Meeting%20Symposia/1511_Imaging_and_analysis_of_3D%5B1%5D.pdf</obo:IAO_0000119>
    </owl:Axiom>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000050 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000050">
        <obo:IAO_0000115 xml:lang="en">A non-barcoded and unamplified cyclic smFISH method (osmFISH) in which the number of targets scales linearly. In osmFISH, each RNA molecule is visualized as a diffraction limited spot after the binding of 20 nt long fluorescently labelled DNA probes. Multiple transcripts are targeted at each round of hybridization, separated by fluorescent color. After image acquisition, the detection probes are removed by formamide melting in preparation for the next round of hybridization.</obo:IAO_0000115>
        <oboInOwl:created_by rdf:resource="http://orcid.org/0000-0002-5260-9315"/>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-07-11T22:15:28Z</oboInOwl:creation_date>
        <rdfs:label>osmFISH</rdfs:label>
    </owl:Class>
    <owl:Axiom>
        <owl:annotatedSource rdf:resource="http://purl.obolibrary.org/obo/TMN_0000050"/>
        <owl:annotatedProperty rdf:resource="http://purl.obolibrary.org/obo/IAO_0000115"/>
        <owl:annotatedTarget xml:lang="en">A non-barcoded and unamplified cyclic smFISH method (osmFISH) in which the number of targets scales linearly. In osmFISH, each RNA molecule is visualized as a diffraction limited spot after the binding of 20 nt long fluorescently labelled DNA probes. Multiple transcripts are targeted at each round of hybridization, separated by fluorescent color. After image acquisition, the detection probes are removed by formamide melting in preparation for the next round of hybridization.</owl:annotatedTarget>
        <obo:IAO_0000119 xml:lang="en">https://doi.org/10.1038/s41592-018-0175-z</obo:IAO_0000119>
    </owl:Axiom>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000051 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000051">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A RNA sequencing assay based on BioSpyder Technologies&apos; Templated Oligo Detection Assay that targets RNAs with detector olives and removes excess probes and enzymatic inhibitors before the first enzymatic step. Correctly hybridized detector olives are ligated, then amplified through primer landing sites that are share among all probes.</obo:IAO_0000115>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">temp-O seq</obo:IAO_0000118>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">templated Oligo sequencing assay</obo:IAO_0000118>
        <oboInOwl:created_by rdf:resource="http://orcid.org/0000-0002-5260-9315"/>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-11-29T21:05:47Z</oboInOwl:creation_date>
        <rdfs:label>temp-O sequencing assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000052 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000052">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A 3D structure determination assay that employs X-ray holographic nano-tomography to determine the three-dimensional structure of an entity.</obo:IAO_0000115>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">XHN</obo:IAO_0000118>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">x-ray holographic nano-tomography</obo:IAO_0000118>
        <oboInOwl:created_by rdf:resource="http://orcid.org/0000-0002-5260-9315"/>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-11-29T21:08:51Z</oboInOwl:creation_date>
        <rdfs:label>x-ray holographic nano-tomography assay</rdfs:label>
    </owl:Class>
    <owl:Axiom>
        <owl:annotatedSource rdf:resource="http://purl.obolibrary.org/obo/TMN_0000052"/>
        <owl:annotatedProperty rdf:resource="http://purl.obolibrary.org/obo/IAO_0000115"/>
        <owl:annotatedTarget rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A 3D structure determination assay that employs X-ray holographic nano-tomography to determine the three-dimensional structure of an entity.</owl:annotatedTarget>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">https://doi.org/10.1038/s41593-020-0704-9</obo:IAO_0000119>
    </owl:Axiom>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000053 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000053">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">A DNA sequencing assay wherein a single DNA polymerase enzyme is affixed at the bottom of a zero-mode waveguide (ZMW) with a single molecule of DNA as a template. The Pacific Biotechnologies version is a single-molecule real-time (SMRT) sequencing assay. The ZMW is a structure that creates an illuminated observation volume that is small enough to observe only a single nucleotide of DNA being incorporated by DNA polymerase. Each of the four DNA bases is attached to one of four different fluorescent dyes. When a nucleotide is incorporated by the DNA polymerase, the fluorescent tag is cleaved off and diffuses out of the observation area of the ZMW where its fluorescence is no longer observable. A detector detects the fluorescent signal of the nucleotide incorporation, and the base call is made according to the corresponding fluorescence of the dye.</obo:IAO_0000115>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">Pac-Bio long-read sequencing assay</obo:IAO_0000118>
        <oboInOwl:created_by rdf:resource="http://orcid.org/0000-0002-5260-9315"/>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-11-29T21:12:31Z</oboInOwl:creation_date>
        <rdfs:label>Pacific Bioscience long-read sequencing assay</rdfs:label>
    </owl:Class>
    


    <!-- http://purl.obolibrary.org/obo/TMN_0000054 -->

    <owl:Class rdf:about="http://purl.obolibrary.org/obo/TMN_0000054">
        <obo:IAO_0000115 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">An imaging assay wherein chromatin accessibility data is collected from cells and enhancers specific to cell types are identified, cloned into recombinant adeno-associated viruses (AAVs) and delivered to the brain, which drive trans gene expression in specific cell types. Then, an image of the marked cell class is generated through fluorescence microscopy.</obo:IAO_0000115>
        <obo:IAO_0000118 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">enhancer virus labelling</obo:IAO_0000118>
        <oboInOwl:created_by rdf:resource="http://orcid.org/0000-0002-5260-9315"/>
        <oboInOwl:creation_date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-11-29T21:14:49Z</oboInOwl:creation_date>
        <rdfs:label>enhancer virus labelling assay</rdfs:label>
    </owl:Class>
    <owl:Axiom>
        <owl:annotatedSource rdf:resource="http://purl.obolibrary.org/obo/TMN_0000054"/>
        <owl:annotatedProperty rdf:resource="http://purl.obolibrary.org/obo/IAO_0000115"/>
        <owl:annotatedTarget rdf:datatype="http://www.w3.org/2001/XMLSchema#string">An imaging assay wherein chromatin accessibility data is collected from cells and enhancers specific to cell types are identified, cloned into recombinant adeno-associated viruses (AAVs) and delivered to the brain, which drive trans gene expression in specific cell types. Then, an image of the marked cell class is generated through fluorescence microscopy.</owl:annotatedTarget>
        <obo:IAO_0000119 rdf:datatype="http://www.w3.org/2001/XMLSchema#string">https://doi.org/10.1016/j.neuron.2021.03.011</obo:IAO_0000119>
    </owl:Axiom>
</rdf:RDF>



<!-- Generated by the OWL API (version 4.5.6) https://github.com/owlcs/owlapi -->